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Droplet Digital PCR

This is a relatively new PCR method in which specific areas of the genetic material can be quantified. The special feature of this method is the division of the reaction into a large number of so-called nanodroplets (droplets), each of which theoretically contains only one DNA/RNA molecule. This strong dilution largely avoids impurities that would lead to a disturbance of the reaction. Nanodroplets containing DNA/RNA show a reaction and are labeled by a dye. Nanodrops that do not contain DNA/RNA are not labeled. The number of labeled and unlabeled droplets is determined. From the ratio, the number of DNA or RNA molecules can be inferred, so that the growth of the fungus and the expression of the biosynthetic genes can be directly followed (molecularly monitored) in the product. This allows very precise statements to be made about the conditions that lead to mycotoxin formation.